We have published the method for rearing Drosophila melanogaster under axenic and gnotobiotic conditions, including how to dechorionate fly embryos hypochlorite and rear the insects under strictly sterile conditions, as well as how to construct associations with defined microbiota, generating gnotobiotic animals. We especially thank Lindy Koyle, Madeline Veloz and Alec Judd for all the great work putting together the video.
Koyle ME, Veloz M, Judd A, Wong A C-N, Newell PD, Douglas AE and Chaston JM 2016. Rearing the fruit fly Drosophila melanogaster under axenic and gnotobiotic conditions. Journal of Visualized Experiments 113, e54219.
The Video is available here